HIC5 Rekombinanter Antikörper

HIC5 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250139E8

Kat-Nr. : 85859-5-PBS

Synonyme

Androgen receptor-associated protein of 55 kDa, ARA55, Hic-5, Hydrogen peroxide-inducible clone 5 protein, TGFB1I1

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  WB analysis of HeLa using 85859-5-RR (same clone as 85859-5-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 85859-5-RR (HIC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85859-5-PBS in a different storage buffer formulation.

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  WB analysis of SKOV-3 using 85859-5-RR (same clone as 85859-5-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 85859-5-RR (HIC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85859-5-PBS in a different storage buffer formulation.

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  WB analysis of rat colon using 85859-5-RR (same clone as 85859-5-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 85859-5-RR (HIC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85859-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85859-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85859-5-RR against Human HIC5 were performed. The affinity constant is 6.15 nM.

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Geprüfte Anwendungen

Produktinformation

85859-5-PBS bindet in WB, Indirect ELISA HIC5 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HIC5 fusion protein Ag0868
• Vollständiger Name: transforming growth factor beta 1 induced transcript 1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC001830
• Gene symbol: HIC5
• Gene ID (NCBI): 7041
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIC5 (Hydrogen Peroxide-Inducible Clone 5), also known as TGFB1I1, ARA55, or TSC-5. It was first identified in 1994 as a gene induced by transforming growth factor-β (TGF-β) or hydrogen peroxide (PMID: 36222304). HIC5 is a member of the Paxillin protein family and functions as a molecular adapter, delivering signals when the cellular adhesion environment changes. Given its role in cancer and fibrotic diseases, HIC5 is a potential therapeutic target. For example, targeting HIC5 or the GR tau2 region could modulate the set of genes regulated by GR, providing opportunities for intervention (PMID: 24591583).