TGFBI/BIGH3 Polyklonaler Antikörper

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• KD/KO Validated

TGFBI/BIGH3 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10188-1-PBS

Synonyme

TGFBI, TGFBI / BIGH3, RGD-containing collagen-associated protein, RGD-CAP, Kerato-epithelin

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  WB analysis using 10188-1-AP (same clone as 10188-1-PBS)

  Mouse eye tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10188-1-AP (same clone as 10188-1-PBS)

  WB result of TGFBI / BIGH3 antibody (10188-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGFBI / BIGH3 transfected HeLa cells. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse eye using 10188-1-AP (same clone as 10188-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10188-1-AP (same clone as 10188-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 10188-1-AP (same clone as 10188-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 10188-1-AP (same clone as 10188-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 10188-1-AP (same clone as 10188-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 10188-1-AP (TGFBI / BIGH3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 10188-1-AP (same clone as 10188-1-PBS)

  IP result of anti-TGFBI / BIGH3 (IP:10188-1-AP, 3ug; Detection:10188-1-AP 1:300) with HeLa cells lysate 1000ug. This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 10188-1-AP (same clone as 10188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 10188-1-AP (TGFBI / BIGH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 10188-1-AP (same clone as 10188-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells, untreated (left) or TGF-β-treated (right), using TGFBI / BIGH3 antibody (10188-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Y79 using 10188-1-AP (same clone as 10188-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Y79 cells using TGFBI / BIGH3 antibody (10188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Y79 using 10188-1-AP (same clone as 10188-1-PBS)

  1X10^6 Y79 cells were intracellularly stained with 0.4 ug Anti-Human TGFBI / BIGH3 (10188-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10188-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10188-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA TGFBI/BIGH3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TGFBI/BIGH3 fusion protein Ag0241
• Vollständiger Name: transforming growth factor, beta-induced, 68kDa
• Berechnetes Molekulargewicht: 683 aa, 75 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC000097
• Gene symbol: TGFBI
• Gene ID (NCBI): 7045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

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