TGFBI / BIGH3 Monoklonaler Antikörper

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TGFBI / BIGH3 Monoklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3E11D11

Kat-Nr. : 60007-1-PBS

Synonyme

TGFBI, 3E11D11, Beta ig h3, Beta ig-h3, BIGH3

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  WB analysis of human kidney using 60007-1-Ig (same clone as 60007-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60007-1-Ig (same clone as 60007-1-PBS)

  IP result of anti-TGFBI / BIGH3 (IP:60007-1-Ig, 4ug; Detection:60007-1-Ig 1:300) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60007-1-Ig (TGFBI / BIGH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using TGFBI / BIGH3 antibody (60007-1-Ig, Clone: 3E11D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 60007-1-Ig (same clone as 60007-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using TGFBI / BIGH3 antibody (60007-1-Ig, Clone: 3E11D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60007-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 60007-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60007-1-Ig against Human TGFBI / BIGH3 were performed. The affinity constant is 1.64 nM.

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Geprüfte Anwendungen

Produktinformation

60007-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA TGFBI / BIGH3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TGFBI / BIGH3 fusion protein Ag0241
• Vollständiger Name: transforming growth factor, beta-induced, 68kDa
• Berechnetes Molekulargewicht: 683 aa, 75 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC000097
• Gene symbol: TGFBI
• Gene ID (NCBI): 7045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).