TGFBI/BIGH3 Rekombinanter Antikörper

TGFBI/BIGH3 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

243108F7

Kat-Nr. : 80805-4-RR

Synonyme

TGFBI, TGFBI / BIGH3, Beta ig h3, Beta ig-h3, BIGH3

---

• 

  WB analysis of HeLa using 80805-4-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 80805-4-RR (beta IG-H3/TGFBI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 80805-4-RR

  A549 cells were subjected to SDS PAGE followed by western blot with 80805-4-RR (beta IG-H3/TGFBI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 80805-4-RR

  Biolayer interferometry (BLl) kinetic assays of 80805-4-RR against Human TGFBI/BIGH3 were performed. The affinity constant is 1.65 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80805-4-RR bindet in WB, ELISA TGFBI/BIGH3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TGFBI/BIGH3 fusion protein Eg1233
• Vollständiger Name: transforming growth factor, beta-induced, 68kDa
• Berechnetes Molekulargewicht: 75kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: NM_000358.3
• Gene symbol: TGFBI
• Gene ID (NCBI): 7045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).