TGFBR1 Polyklonaler Antikörper

TGFBR1 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 30117-1-AP

Synonyme

TGFβ RI, AAT5, Activin A receptor type II-like protein kinase of 53kD, Activin receptor like kinase 5, Activin receptor-like kinase 5

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  WB analysis using 30117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30117-1-AP (TGFBR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 30117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30117-1-AP (TGFBR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 30117-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30117-1-AP (TGFBR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 30117-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30117-1-AP (TGFBR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse ovary using 30117-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using TGFBR1 antibody (30117-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, MDA-MB-231-Zellen, Mauslebergewebe, NCI-H1299-Zellen, Rattenlebergewebe, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Eierstockgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 3 publications below

Produktinformation

30117-1-AP bindet in WB, IHC, IF-P, ELISA TGFBR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TGFBR1 fusion protein Ag31620
• Vollständiger Name: transforming growth factor, beta receptor 1
• Berechnetes Molekulargewicht: 56KD
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: NM_004612
• Gene symbol: TGFBR1
• Gene ID (NCBI): 7046
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGFBR1 (TGF-beta receptor type-1) encodes a serine/threonine kinase receptor for transforming growth factor-beta. TGFB1, TGFB2 and TGFB3 signals are transduced from the cell surface to the cytoplasm and regulate lots of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Mutations in both TGFBR2 and TGFBR1 were associated with early onset and aggressive thoracic aortic disease with MFS-like skeletal features, but also hypertelorism, craniosynostosis, developmental delay, cleft palate and bifid uvula, congenital heart disease and aneurysms, and dissections throughout the arterial tree with marked arterial tortuosity (PMID: 15731757, PMID: 27879313).

Publikationen

Species	Application	Title
mouse	IF	Theranostics; Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction; Authors - Meng Gao
mouse	WB	J Nanobiotechnology; Determining M2 macrophages content for the anti-tumor effects of metal-organic framework-encapsulated pazopanib nanoparticles in breast cancer; Authors - Zhijie Xu
mouse	IF	Nat Commun; Targeting the transmembrane cytokine co-receptor neuropilin-1 in distal tubules improves renal injury and fibrosis; Authors - Yinzheng Li
human	WB	Mol Cell Biochem; Lnc PVT1 facilitates TGF-β1-induced human cardiac fibroblast activation in vitro and ISO-induced myocardial fibrosis in vivo through regulating MYC; Authors - Juan Wang
human	WB	Front Pharmacol; Isoliensinine suppressed gastric cancer cell proliferation and migration by targeting TGFBR1 to regulate TGF-β-smad signaling pathways; Authors - Jinda Hu
human	WB	Epigenetics; ANRIL upregulates TGFBR1 to promote idiopathic pulmonary fibrosis in TGF-β1-treated lung fibroblasts via sequestering let-7d-5p; Authors - Weidong Wu