TGFBR1 Polyklonaler Antikörper

TGFBR1 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30117-1-PBS

Synonyme

TGFβ RI, AAT5, Activin A receptor type II-like protein kinase of 53kD, Activin receptor like kinase 5, Activin receptor-like kinase 5

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  WB analysis using 30117-1-AP (same clone as 30117-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30117-1-AP (TGFBR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30117-1-PBS in a different storage buffer formulation.

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  WB analysis using 30117-1-AP (same clone as 30117-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30117-1-AP (TGFBR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30117-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 30117-1-AP (same clone as 30117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30117-1-AP (TGFBR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30117-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 30117-1-AP (same clone as 30117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30117-1-AP (TGFBR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30117-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse ovary using 30117-1-AP (same clone as 30117-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using TGFBR1 antibody (30117-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30117-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30117-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA TGFBR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TGFBR1 fusion protein Ag31620
• Vollständiger Name: transforming growth factor, beta receptor 1
• Berechnetes Molekulargewicht: 56KD
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: NM_004612
• Gene symbol: TGFBR1
• Gene ID (NCBI): 7046
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGFBR1 (TGF-beta receptor type-1) encodes a serine/threonine kinase receptor for transforming growth factor-beta. TGFB1, TGFB2 and TGFB3 signals are transduced from the cell surface to the cytoplasm and regulate lots of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Mutations in both TGFBR2 and TGFBR1 were associated with early onset and aggressive thoracic aortic disease with MFS-like skeletal features, but also hypertelorism, craniosynostosis, developmental delay, cleft palate and bifid uvula, congenital heart disease and aneurysms, and dissections throughout the arterial tree with marked arterial tortuosity (PMID: 15731757, PMID: 27879313).