TGFBR1 Rekombinanter Antikörper

TGFBR1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241441A11

Kat-Nr. : 84453-1-RR

Synonyme

Activin receptor-like kinase 5, Activin receptor like kinase 5, Activin A receptor type II-like protein kinase of 53kD, AAT5, 241441A11

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  WB analysis using 84453-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84453-1-RR (TGFBR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 84453-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84453-1-RR (TGFBR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 84453-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84453-1-RR (TGFBR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 84453-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84453-1-RR (TGFBR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84453-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84453-1-RR against Human TGFBR1 were performed. The affinity constant is 67.5 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NCI-H1299-Zellen, A549-Zellen, MDA-MB-231-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84453-1-RR bindet in WB, IHC, ELISA TGFBR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TGFBR1 fusion protein Ag31620
• Vollständiger Name: transforming growth factor, beta receptor 1
• Berechnetes Molekulargewicht: 56KD
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: NM_004612
• Gene symbol: TGFBR1
• Gene ID (NCBI): 7046
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGFBR1 (TGF-beta receptor type-1) encodes a serine/threonine kinase receptor for transforming growth factor-beta. TGFB1, TGFB2 and TGFB3 signals are transduced from the cell surface to the cytoplasm and regulate lots of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Mutations in both TGFBR2 and TGFBR1 were associated with early onset and aggressive thoracic aortic disease with MFS-like skeletal features, but also hypertelorism, craniosynostosis, developmental delay, cleft palate and bifid uvula, congenital heart disease and aneurysms, and dissections throughout the arterial tree with marked arterial tortuosity (PMID: 15731757, PMID: 27879313).