TGFBR2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

TGFBR2 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(53)

CloneNo.

2D5H7

Rezensionen (1)

Kat-Nr. : 66636-1-Ig

Synonyme

2D5H7, EC:2.7.11.30, TbetaR-II, TGF beta receptor type 2, TGF-beta receptor type II

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• 

  WB analysis using 66636-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66636-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of HEK-293T using 66636-1-Ig

  WB result of TGFBR2 antibody (66636-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGFBR2 transfected HEK-293T cells.

• 

  WB analysis of U2OS using 66636-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66636-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66636-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66636-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66636-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66636-1-Ig (TGFBR2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66636-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66636-1-Ig (TGFBR2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293T-Zellen, HepG2-Zellen, K-562-Zellen, L02-Zellen, NCCIT-Zellen, NIH/3T3-Zellen, THP-1-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 46 publications below
• IHC: See 6 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Produktinformation

66636-1-Ig bindet in WB, IHC, IF, IP, CoIP, ELISA TGFBR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TGFBR2 fusion protein Ag25773
• Vollständiger Name: transforming growth factor, beta receptor II (70/80kDa)
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 65-80 kDa
• GenBank-Zugangsnummer: BC040499
• Gene symbol: TGFBR2
• Gene ID (NCBI): 7048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). The calculated molecular weight of TGFBR2 is 65 kDa. It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.

Publikationen

Species	Application	Title
mouse	WB	Cell Rep Med; Engineering irradiated tumor-derived microparticles as personalized vaccines to enhance anti-tumor immunity; Authors - Yajie Sun
human	WB	Dev Cell; DYRK1A-TGF-β signaling axis determines sensitivity to OXPHOS inhibition in hepatocellular carcinoma; Authors - Ying Cao ; KD Validated
mouse	WB,IF	Mol Ther Nucleic Acids; Exploring the therapeutic potential of sγPNA-141: Pharmacodynamics and mechanistic insights during ischemic stroke recovery; Authors - Sanjeev Kumar Yadav
human	WB	Cell Rep; TGF-β1-mediated intercellular signaling fuels cooperative cellular invasion; Authors - Tala O Khatib
rat,mouse	WB,IHC,IF	Acta Pharmacol Sin; GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy; Authors - Ji-Chao Wu
rat	WB,IP	Br J Pharmacol; Inhibition of HSP90 S-nitrosylation Alleviates Cardiac Fibrosis via TGFβ/SMAD3 Signaling Pathway.; Authors - Xiyue Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (08-15-2023)

This antibody worked well for labeling TGF beta receptor type 2 in mouse kidney. We were able to acquire robust immunofluorescence signals with this KO validated antibody.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Kidney tissue