TGFBR2 Monoklonaler Antikörper

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TGFBR2 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D5H7

Kat-Nr. : 66636-1-PBS

Synonyme

2D5H7, EC:2.7.11.30, TbetaR-II, TGF beta receptor type 2, TGF-beta receptor type II

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  WB analysis using 66636-1-Ig (same clone as 66636-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis using 66636-1-Ig (same clone as 66636-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 66636-1-Ig (same clone as 66636-1-PBS)

  WB result of TGFBR2 antibody (66636-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGFBR2 transfected HEK-293T cells. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 66636-1-Ig (same clone as 66636-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66636-1-Ig (same clone as 66636-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66636-1-Ig (same clone as 66636-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66636-1-Ig (same clone as 66636-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66636-1-Ig (same clone as 66636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66636-1-Ig (TGFBR2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66636-1-Ig (same clone as 66636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66636-1-Ig (TGFBR2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66636-1-PBS bindet in WB, IHC, ELISA TGFBR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TGFBR2 fusion protein Ag25773
• Vollständiger Name: transforming growth factor, beta receptor II (70/80kDa)
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 65-80 kDa
• GenBank-Zugangsnummer: BC040499
• Gene symbol: TGFBR2
• Gene ID (NCBI): 7048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). The calculated molecular weight of TGFBR2 is 65 kDa. It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.