TGFBR2 Rekombinanter Antikörper

TGFBR2 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250238A7

Kat-Nr. : 98438-1-PBS

Synonyme

EC:2.7.11.30, TbetaR-II, TGF beta receptor type 2, TGF-beta receptor type II, TGF-beta receptor type-2

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  FC experiment of human PBMCs using 98438-1-RR (same clone as 98438-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TGFBR2 Rabbit RecAb (98438-1-RR, Clone: 250238A7) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98438-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98438-1-RR (same clone as 98438-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TGFBR2 Rabbit RecAb (98438-1-RR, Clone: 250238A7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98438-1-PBS in a different storage buffer formulation.

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  FC experiment of human peripheral blood leukocytes using 98438-1-RR (same clone as 98438-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human TGFBR2 Rabbit RecAb (98438-1-RR, Clone: 250238A7) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98438-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98438-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 98438-1-PBS against Human TGFBR2 was performed. The affinity constant is 0.937 nM.

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Geprüfte Anwendungen

Produktinformation

98438-1-PBS bindet in FC TGFBR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TGFBR2 fusion protein Eg2943
• Vollständiger Name: transforming growth factor, beta receptor II (70/80kDa)
• Berechnetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: NM_003242.5
• Gene symbol: TGFBR2
• Gene ID (NCBI): 7048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 is a serine/threonine kinase and the primary ligand-binding receptor. After binding of the ligand TGF-β1, TGFBR2 forms a heterodimer complex with TGFBR1, causing conformational changes and signal propagation via canonical (SMAD-dependent) and non-canonical (SMAD-independent) routes. Upon translocation into the nucleus, activated SMAD complexes can interact with various transcription factors to control target gene expression (PMID: 31454892).