TGIF2 Polyklonaler Antikörper

TGIF2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11522-1-AP

Synonyme

5 TG 3 interacting factor 2, Homeobox protein TGIF2, TGFB induced factor 2, TGFB induced factor homeobox 2, TGIF2

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Galerie der Validierungsdaten

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  WB analysis using 11522-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11522-1-AP (TGIF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 11522-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11522-1-AP (TGIF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11522-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11522-1-AP (TGIF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 11522-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using TGIF2 antibody (11522-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Daudi-Zellen, HL-60-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Caco-2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

11522-1-AP bindet in WB, IHC, IF, ELISA TGIF2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TGIF2 fusion protein Ag2085
• Vollständiger Name: TGFB-induced factor homeobox 2
• Berechnetes Molekulargewicht: 237 aa, 26 kDa
• Beobachtetes Molekulargewicht: 28 kDa and 30 kDa
• GenBank-Zugangsnummer: BC012816
• Gene symbol: TGIF2
• Gene ID (NCBI): 60436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transforming growth factor-beta-induced factor 2(TGIF2), is a member of a subfamily of transcription factor with a TALE(3-amino acid loop extension), an atypical homeodomain. It also contains a proline-rich region, which is a putative SH3 domain-binding site and suggested involve in transcriptional regulation. It is highly expressed in the heart, kidney, and testis, but low in the brain and prostate. Through binding directly to the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins, TGIF2 acts as a transcriptional repressor. The calculated molecular weight of TGIF2 is 26 kDa, but the phosphorylated TGIF2 is about 30-35 kDa protein. This antibody detected two forms of bands of about 28 and 30 kDa, and the upper band appears to result from increased phosphorylation (PMID: 11427533).

Publikationen

Species	Application	Title
mouse	WB	Cell Rep; Tgif1 Counterbalances the Activity of Core Pluripotency Factors in Mouse Embryonic Stem Cells.; Authors - Bum-Kyu Lee
human	WB	Biochem J; The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome.; Authors - Agata Rozanska
human	WB	Exp Ther Med; Molecular mechanisms in vascular injury induced by hypertension: Expression and role of microRNA-34a.; Authors - Siguan Liu
mouse	WB	Brain Behav; Downregulation of TGIF2 is possibly correlated with neuronal apoptosis and autism-like symptoms in mice.; Authors - Jing Lei