TGM2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TGM2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(27)

Rezensionen (1)

Kat-Nr. : 15100-1-AP

Synonyme

Erythrocyte transglutaminase, TG(C), TG2, TGase 2, TGase C

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  WB analysis using 15100-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15100-1-AP (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15100-1-AP

  WB result of TGM2 antibody (15100-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGM2 transfected HepG2 cells.

• 

  WB analysis of HUVEC using 15100-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 15100-1-AP (TGM2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15100-1-AP

  IP result of anti-TGM2 (IP:15100-1-AP, 4ug; Detection:15100-1-AP 1:400) with HeLa cells lysate 1200ug.

• 

  IHC staining of human liver cancer using 15100-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15100-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15100-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15100-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, A549-Zellen, BxPC-3-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 26 publications below
• IHC: See 10 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

15100-1-AP bindet in WB, IHC, IF, IP, ChIP, ELISA TGM2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TGM2 fusion protein Ag7439
• Vollständiger Name: transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 77-80 kDa
• GenBank-Zugangsnummer: BC003551
• Gene symbol: TGM2
• Gene ID (NCBI): 7052
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transglutaminase 2 (TGM2) is a ubiquitous and multifunctional calcium-dependent enzyme belonging to the transglutaminase family. It is best known for its canonical activity of catalyzing the cross-linking of proteins by forming stable ε-(γ-glutamyl)lysine isopeptide bonds, which contributes to extracellular matrix stabilization and wound healing. Beyond this, TGM2 exhibits GTPase activity, allowing it to function as a signaling G-protein in intracellular processes. It is implicated in a wide range of physiological functions, including cell adhesion, proliferation, and apoptosis, as well as pathological conditions such as celiac disease, fibrosis, neurodegenerative disorders, and cancer metastasis, where its dysregulated expression often contributes to disease progression.

Publikationen

Species	Application	Title
		Nature; Autoimmune amelogenesis imperfecta in patients with APS-1 and coeliac disease; Authors - Yael Gruper
human,canine	IF	Nat Genet; New genes helped acorn barnacles adapt to a sessile lifestyle; Authors - Zhaofang Han
human	WB,IF	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
human	WB,IF	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
human	WB,IHC	Cell Oncol (Dordr); Transglutaminases are oncogenic biomarkers in human cancers and therapeutic targeting of TGM2 blocks chemoresistance and macrophage infiltration in pancreatic cancer; Authors - Shan Zhang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jon (Verified Customer) (02-15-2022)

DILuted as suggested in %5 milk TBST blocking buffer. We have had problems with other TGM2 antibodies from other companies, but this one seemed no work better and yield a better signal. However, the apparent molecular weight of the TGM2 band appeared to be at a much lower MW than it is supposed to.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: EndoCßH1 human pancreatic beta cell line

TGM2 Antibody Western Blot validation (1:1000 dilution) in EndoCßH1 human pancreatic beta cell line (Cat no:15100-1-AP)