TGM2 Polyklonaler Antikörper

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• KD/KO Validated

TGM2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15100-1-PBS

Synonyme

Erythrocyte transglutaminase, TG(C), TG2, TGase 2, TGase C

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  WB analysis of HepG2 using 15100-1-AP (same clone as 15100-1-PBS)

  WB result of TGM2 antibody (15100-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGM2 transfected HepG2 cells. This data was developed using the same antibody clone with 15100-1-PBS in a different storage buffer formulation.

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  WB analysis using 15100-1-AP (same clone as 15100-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15100-1-AP (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15100-1-PBS in a different storage buffer formulation.

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  WB analysis of HUVEC using 15100-1-AP (same clone as 15100-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 15100-1-AP (TGM2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15100-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15100-1-AP (same clone as 15100-1-PBS)

  IP result of anti-TGM2 (IP:15100-1-AP, 4ug; Detection:15100-1-AP 1:400) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 15100-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15100-1-AP (same clone as 15100-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15100-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15100-1-AP (same clone as 15100-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15100-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15100-1-AP (same clone as 15100-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15100-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15100-1-AP (same clone as 15100-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15100-1-AP (TGM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15100-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15100-1-PBS bindet in WB, IHC, IP, Indirect ELISA TGM2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TGM2 fusion protein Ag7439
• Vollständiger Name: transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 77-80 kDa
• GenBank-Zugangsnummer: BC003551
• Gene symbol: TGM2
• Gene ID (NCBI): 7052
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transglutaminase 2 (TGM2) is a ubiquitous and multifunctional calcium-dependent enzyme belonging to the transglutaminase family. It is best known for its canonical activity of catalyzing the cross-linking of proteins by forming stable ε-(γ-glutamyl)lysine isopeptide bonds, which contributes to extracellular matrix stabilization and wound healing. Beyond this, TGM2 exhibits GTPase activity, allowing it to function as a signaling G-protein in intracellular processes. It is implicated in a wide range of physiological functions, including cell adhesion, proliferation, and apoptosis, as well as pathological conditions such as celiac disease, fibrosis, neurodegenerative disorders, and cancer metastasis, where its dysregulated expression often contributes to disease progression.