TGM2 Monoklonaler Antikörper

TGM2 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D4C11

Kat-Nr. : 68006-1-PBS

Synonyme

2D4C11, Erythrocyte transglutaminase, TG(C), TG2, TGase 2

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  WB analysis of HeLa using 68006-1-Ig (same clone as 68006-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68006-1-Ig (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

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  WB analysis of HUVEC using 68006-1-Ig (same clone as 68006-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 68006-1-Ig (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68006-1-Ig (same clone as 68006-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68006-1-Ig (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 68006-1-Ig (same clone as 68006-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 68006-1-Ig (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 68006-1-Ig (same clone as 68006-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68006-1-Ig (TGM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68006-1-Ig (same clone as 68006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68006-1-Ig (TGM2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 68006-1-Ig (same clone as 68006-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TGM2 antibody (68006-1-Ig, Clone: 2D4C11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68006-1-Ig (same clone as 68006-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TGM2 (68006-1-Ig, Clone:2D4C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68006-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51456-1

  Cytometric bead array sample test of MP51456-1, TGM2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68006-1-PBS. Detection antibody: 68006-2-PBS.

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  Cytometric bead array standard curve of MP51456-1

  Cytometric bead array standard curve of MP51456-1, TGM2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68006-1-PBS. Detection antibody: 68006-2-PBS. Standard:Ag7462. Range: 3.125-400 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68006-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA TGM2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TGM2 fusion protein Ag7462
• Vollständiger Name: transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC003551
• Gene symbol: TGM2
• Gene ID (NCBI): 7052
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transglutaminase 2 (TGM2) is a ubiquitous and multifunctional calcium-dependent enzyme belonging to the transglutaminase family. It is best known for its canonical activity of catalyzing the cross-linking of proteins by forming stable ε-(γ-glutamyl)lysine isopeptide bonds, which contributes to extracellular matrix stabilization and wound healing. Beyond this, TGM2 exhibits GTPase activity, allowing it to function as a signaling G-protein in intracellular processes. It is implicated in a wide range of physiological functions, including cell adhesion, proliferation, and apoptosis, as well as pathological conditions such as celiac disease, fibrosis, neurodegenerative disorders, and cancer metastasis, where its dysregulated expression often contributes to disease progression.