TGN46 Monoklonaler Antikörper

TGN46 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F6D5

Kat-Nr. : 66477-1-PBS

Synonyme

TGOLN2, 1F6D5, hTGN46, hTGN48, hTGN51

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  WB analysis of HeLa using 66477-1-Ig (same clone as 66477-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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  WB analysis using 66477-1-Ig (same clone as 66477-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66477-1-Ig (same clone as 66477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66477-1-Ig (same clone as 66477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66477-1-PBS bindet in WB, IHC, Indirect ELISA TGN46 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TGN46 fusion protein Ag4470
• Vollständiger Name: trans-golgi network protein 2
• Berechnetes Molekulargewicht: 447 aa, 47 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC028219
• Gene symbol: TGN46
• Gene ID (NCBI): 10618
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.