TGN46 Monoklonaler Antikörper

TGN46 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

1F6D5

Rezensionen (1)

Kat-Nr. : 66477-1-Ig

Synonyme

TGOLN2, 1F6D5, hTGN46, hTGN48, hTGN51

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  WB analysis of HeLa using 66477-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66477-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66477-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, L02-Zellen, LNCaP-Zellen, Hausschwein-Lebergewebe, SMMC-7721-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66477-1-Ig bindet in WB, IHC, ELISA TGN46 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TGN46 fusion protein Ag4470
• Vollständiger Name: trans-golgi network protein 2
• Berechnetes Molekulargewicht: 447 aa, 47 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC028219
• Gene symbol: TGN46
• Gene ID (NCBI): 10618
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

Publikationen

Species	Application	Title
		Sci Adv; Isocyanic acid-mediated NLRP3 carbamoylation reduces NLRP3-NEK7 interaction and limits inflammasome activation; Authors - Zhenxing Zhang
human		Arterioscler Thromb Vasc Biol; IL-1β Induces LDL Transcytosis by a Novel Pathway Involving LDLR and Rab27a; Authors - Erika Jang
pig		J Virol; Porcine deltacoronavirus accessory protein NS6 harnesses VPS35-mediated retrograde trafficking to facilitate efficient viral infection; Authors - Puxian Fang
mouse		J Cell Physiol; Locational and functional characterization of PI4KB in the mouse embryo; Authors - Haoyu Zhao
mouse		Int J Biol Sci; Annexin A5 Derived from Cell-free Fat Extract Attenuates Osteoarthritis via Macrophage Regulation; Authors - Zhuoxuan Jia
human	WB	Mol Metab; Tetraspanin7 in Adipose Tissue Remodeling and Its Impact on Metabolic Health; Authors - Shino Nemoto

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Karla (Verified Customer) (12-20-2022)

Proteintech has the best for IMF. Made my images clear and was picked up easily on the microscope.

• Applications: Immunofluorescence