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TGN46 Monoklonaler Antikörper

TGN46 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F6D5

Kat-Nr. : 66477-1-Ig

Synonyme

Golgi marker, TGN38, TGN38 homolog, TGN46, TGN48, TGN51, TGOLN2, trans golgi network protein 2, TTGN2

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of HeLa cells using TGN46 Monoclonal antibody (66477-1-Ig)

    WB analysis of HeLa using 66477-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TGN46 Monoclonal antibody (66477-1-Ig)

    WB analysis using 66477-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TGN46 Monoclonal antibody (66477-1-Ig)

    IHC staining of human breast cancer using 66477-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TGN46 Monoclonal antibody (66477-1-Ig)

    IHC staining of human breast cancer using 66477-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TGN46 Monoclonal antibody (66477-1-Ig)

    IHC staining of human liver cancer using 66477-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TGN46 Monoclonal antibody (66477-1-Ig)

    IHC staining of human liver cancer using 66477-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, Hausschwein-Lebergewebe, HeLa-Zellen, HepG2-Zellen, L02-Zellen, LNCaP-Zellen, SMMC-7721-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunhistochemie (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66477-1-Ig bindet in WB, IHC, ELISA TGN46 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen TGN46 fusion protein Ag4470
Vollständiger Name trans-golgi network protein 2
Berechnetes Molekulargewicht 447 aa, 47 kDa
Beobachtetes Molekulargewicht 90 kDa
GenBank-ZugangsnummerBC028219
Gene symbol TGOLN2
Gene ID (NCBI) 10618
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

Protokolle

Produktspezifische Protokolle
WB protocol for TGN46 antibody 66477-1-IgProtokoll herunterladen
IHC protocol for TGN46 antibody 66477-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Karla (Verified Customer) (12-20-2022)

Proteintech has the best for IMF. Made my images clear and was picked up easily on the microscope.

  • Applications: Immunofluorescence