TGN46 Rekombinanter Antikörper

TGN46 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

230376D6

Rezensionen (1)

Kat-Nr. : 83099-2-RR

Synonyme

TGOLN2,TGN46, TGOLN2, TGN38 homolog, TGN38, hTGN51

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  WB analysis using 83099-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83099-2-RR (TGN46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 83099-2-RR

  IP result of anti-TGN46 (IP:83099-2-RR, 4ug; Detection:83099-2-RR 1:2000) with HeLa cells lysate 980 ug.

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  IHC staining of human stomach cancer using 83099-2-RR

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 83099-2-RR (TGN46 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 83099-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGN46 antibody (83099-2-RR, Clone: 230376D6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 83099-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGOLN2,TGN46 antibody (83099-2-RR, Clone: 230376D6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 83099-2-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug TGOLN2,TGN46 Recombinant antibody (83099-2-RR, Clone:230376D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83099-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83099-2-RR against Human TGOLN2,TGN46 were performed. The affinity constant is 0.633 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, humanes Nierengewebe, PC-12-Zellen, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83099-2-RR bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA TGN46 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TGN46 fusion protein Ag4470
• Vollständiger Name: trans-golgi network protein 2
• Berechnetes Molekulargewicht: 447 aa, 47 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC028219
• Gene symbol: TGN46
• Gene ID (NCBI): 10618
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jacob (Verified Customer) (07-25-2025)

Works for IF staining beautifully with PFA-fixed cells. Very little background and nice Golgi localization.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa

TGN46 Antibody Immunofluorescence validation (1:100 dilution) in HeLa (Cat no:83099-2-RR)