TH Polyklonaler Antikörper

TH Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(198)

Rezensionen (4)

Kat-Nr. : 25859-1-AP

Synonyme

tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH

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  WB analysis using 25859-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25859-1-AP (TH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25859-1-AP

  IP result of anti-TH (IP:25859-1-AP, 4ug; Detection:25859-1-AP 1:20000) with mouse brain tissue lysate 2120 ug.

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  IHC staining of mouse brain using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 25859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 25859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 25859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 25859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 25859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using TH antibody (25859-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), MAP2 antibody (67015-1-Ig, Clone: 1C3E6, red).

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  IF Staining of PC-12 using 25859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using TH antibody (25859-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF-Fro: Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 120 publications below
• IHC: See 65 publications below
• IF: See 83 publications below
• ELISA: See 1 publications below

Produktinformation

25859-1-AP bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA TH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte, Zebrafisch, Mongolische Wüstenrennmaus (Gerbil)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TH fusion protein Ag23075
• Vollständiger Name: tyrosine hydroxylase
• Berechnetes Molekulargewicht: 528 aa, 59 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC104967
• Gene symbol: TH
• Gene ID (NCBI): 7054
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoforms produced by alternative splicing with the MW from 44 kDa to 58 kDa.

Publikationen

Species	Application	Title
rat	WB,IHC	Signal Transduct Target Ther; Identifying genetic targets in clinical subtypes of Parkinson's disease for optimizing pharmacological treatment strategies; Authors - Dewen Kong
mouse	IF	Neuron; Gut-induced alpha-Synuclein and Tau propagation initiate Parkinson's and Alzheimer's disease co-pathology and behavior impairments; Authors - Jie Xiang
mouse	WB,IF	Adv Sci (Weinh); OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model; Authors - Xiaomeng Song
mouse	IF	Sci Adv; Lamellar Schwann cells in the Pacinian corpuscle potentiate vibration perception; Authors - Yuh-Tarng Chen
mouse	WB	Nat Commun; Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance.; Authors - Yan-Ting Chen
mouse	IF	Nat Commun; NAc-VTA circuit underlies emotional stress-induced anxiety-like behavior in the three-chamber vicarious social defeat stress mouse model.; Authors - Guangjian Qi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (01-05-2024)

This antibody worked well for immunolabeling on mouse brain tissue sections

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse brain

FH

Raphael (Verified Customer) (08-02-2021)

This antibody is good for western blot. The dilution at 1/1000 was too strong but at 1/2000 it works perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: Rat brain

TH Antibody Western Blot validation (1/2000 dilution) in Rat brain (Cat no:25859-1-AP)

FH

Teresa (Verified Customer) (11-03-2017)

Standard protocol, blocked with 1% goat serum in TBS, heat-mediated antigen retrieval

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: wildtype mouse brain

TH Antibody Immunofluorescence validation (1:200 dilution) in wildtype mouse brain (Cat no:25859-1-AP)

FH

Teresa (Verified Customer) (10-16-2017)

Signal was strong and specific. Could probably dilute further.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse brain

TH Antibody Immunohistochemistry validation (1:200 dilution) in mouse brain (Cat no:25859-1-AP)