TH Polyklonaler Antikörper

TH Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

104

Rezensionen (4)

Kat-Nr. : 25859-1-AP

Synonyme

TH, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, tyrosine hydroxylase

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Galerie der Validierungsdaten

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  WB analysis using 25859-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25859-1-AP (TH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25859-1-AP

  IP result of anti-TH (IP:25859-1-AP, 4ug; Detection:25859-1-AP 1:1000) with mouse brain tissue lysate 4000 ug.

• 

  IHC staining of mouse brain using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 25859-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 25859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 25859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 25859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 25859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 25859-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 25859-1-AP (TH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Mausherzgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:5000-1:20000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 63 publications below
• IHC: See 33 publications below
• IF: See 39 publications below
• ELISA: See 1 publications below

Produktinformation

25859-1-AP bindet in WB, IP, IHC, IF, ELISA TH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte, Zebrafisch, Mongolische Wüstenrennmaus (Gerbil)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TH fusion protein Ag23075
• Vollständiger Name: tyrosine hydroxylase
• Berechnetes Molekulargewicht: 528 aa, 59 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC104967
• Gene symbol: TH
• Gene ID (NCBI): 7054
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoforms produced by alternative splicing with the MW from 44 kDa to 58 kDa.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance.; Authors - Yan-Ting Chen
mouse	IF	Nat Commun; NAc-VTA circuit underlies emotional stress-induced anxiety-like behavior in the three-chamber vicarious social defeat stress mouse model.; Authors - Guangjian Qi
mouse	IF	Nat Commun; Locus coeruleus-CA1 projections are involved in chronic depressive stress-induced hippocampal vulnerability to transient global ischaemia.; Authors - Qian Zhang
mouse	WB	Brain; Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.; Authors - Yun Xia
mouse	IHC	Acta Biomater; Biomaterial based strategies to reconstruct the nigrostriatal pathway in organotypic slice co-cultures.; Authors - Buket Ucar
mouse	IHC	Redox Biol; Targeting mitochondria-inflammation circle by renal denervation reduces atheroprone endothelial phenotypes and atherosclerosis.; Authors - Zhuqing Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenzo (Verified Customer) (01-05-2024)

This antibody worked well for immunolabeling on mouse brain tissue sections

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse brain

FH

Raphael (Verified Customer) (08-02-2021)

This antibody is good for western blot. The dilution at 1/1000 was too strong but at 1/2000 it works perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: Rat brain

TH Antibody Western Blot validation (1/2000 dilution) in Rat brain (Cat no:25859-1-AP)

FH

Teresa (Verified Customer) (11-03-2017)

Standard protocol, blocked with 1% goat serum in TBS, heat-mediated antigen retrieval

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: wildtype mouse brain

TH Antibody Immunofluorescence validation (1:200 dilution) in wildtype mouse brain (Cat no:25859-1-AP)

FH

Teresa (Verified Customer) (10-16-2017)

Signal was strong and specific. Could probably dilute further.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse brain

TH Antibody Immunohistochemistry validation (1:200 dilution) in mouse brain (Cat no:25859-1-AP)

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