TH Polyklonaler Antikörper

TH Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25859-1-PBS

Synonyme

tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH

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  WB analysis using 25859-1-AP (same clone as 25859-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25859-1-AP (TH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

  IP result of anti-TH (IP:25859-1-AP, 4ug; Detection:25859-1-AP 1:20000) with mouse brain tissue lysate 2120 ug. This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 25859-1-AP (same clone as 25859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using TH antibody (25859-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), MAP2 antibody (67015-1-Ig, Clone: 1C3E6, red). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 25859-1-AP (same clone as 25859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using TH antibody (25859-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25859-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, Indirect ELISA TH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TH fusion protein Ag23075
• Vollständiger Name: tyrosine hydroxylase
• Berechnetes Molekulargewicht: 528 aa, 59 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC104967
• Gene symbol: TH
• Gene ID (NCBI): 7054
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoforms produced by alternative splicing with the MW from 44 kDa to 58 kDa.