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TH Monoklonaler Antikörper

TH Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(30)

CloneNo.

2H7B7

Kat-Nr. : 66334-1-Ig

Synonyme

tyrosine hydroxylase, 2H7B7, DYT14, DYT5b, EC:1.14.16.2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBSH-SY5Y-Zellen
Erfolgreiche Detektion in IF/ICCSH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66334-1-Ig bindet in WB, IHC, IF/ICC, ELISA TH und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen TH fusion protein Ag23075
Vollständiger Name tyrosine hydroxylase
Berechnetes Molekulargewicht 528 aa, 59 kDa
Beobachtetes Molekulargewicht 55 kDa
GenBank-ZugangsnummerBC104967
Gene symbol TH
Gene ID (NCBI) 7054
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of DA and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for DA and noradrenergic neurons.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for TH antibody 66334-1-IgProtokoll herunterladen
IF protocol for TH antibody 66334-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
WB,IF

J Ginseng Res

The necessity of eliminating the interference of panaxatriol saponins to maximize the preventive effect of panaxadiol saponins against Parkinson's disease in rats

Authors - Yanwei Wang
humanWB

Mol Oncol

Norepinephrine-CREB1-miR-373 axis promotes progression of colon cancer.

Authors - Jia Han
WB

Front Cell Dev Biol

RAB39B Deficiency Impairs Learning and Memory Partially Through Compromising Autophagy.

Authors - Mengxi Niu
humanIF

Antioxidants (Basel)

Mitochondrial Calcium-Triggered Oxidative Stress and Developmental Defects in Dopaminergic Neurons Differentiated from Deciduous Teeth-Derived Dental Pulp Stem Cells with MFF Insufficiency

Authors - Xiao Sun
WB,IF

Bone Joint Res

High-intensity interval training improves fatty infiltration in the rotator cuff through the β3 adrenergic receptor in mice

Authors - Hecheng Zhou
IHC

Biomed Pharmacother

Nardosinone regulates the slc38a2 gene to alleviate Parkinson's symptoms in rats through the GABAergic synaptic and cAMP pathways.

Authors - Li-Hua Bian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Angie (Verified Customer) (01-20-2025)

Overnight incubation of primary antibody (used at 1:200) at 4 °C followed by 1 h incubation of secondary antibody (Alexa Fluor Donkey anti-mouse 594, 1:500) at room temperature.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Differentiated SHSY-5Y
TH Antibody Immunofluorescence validation (1:200 dilution) in Differentiated SHSY-5Y (Cat no:66334-1-Ig)