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TH Monoklonaler Antikörper

TH Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H7B7

Kat-Nr. : 66334-1-PBS

Synonyme

tyrosine hydroxylase, 2H7B7, DYT14, DYT5b, EC:1.14.16.2

Filter:
  • Western Blot (WB) analysis of SH-SY5Y cells using TH Monoclonal antibody (66334-1-Ig)

    WB analysis of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

    WB result of TH antibody (66334-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TH transfected SH-SY5Y cells. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using TH Monoclonal antibody (66334-1-Ig)

    WB analysis of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using TH Monoclonal antibody (66334-1-Ig)

    IF Staining of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66334-1-Ig(TH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66334-1-PBS bindet in WB, IF/ICC, Indirect ELISA TH und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen TH fusion protein Ag23075
Vollständiger Name tyrosine hydroxylase
Berechnetes Molekulargewicht 528 aa, 59 kDa
Beobachtetes Molekulargewicht 55 kDa
GenBank-ZugangsnummerBC104967
Gene symbol TH
Gene ID (NCBI) 7054
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of DA and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for DA and noradrenergic neurons.