TH Rekombinanter Antikörper

TH Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250146D7

Kat-Nr. : 85929-5-PBS

Synonyme

tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH

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  WB analysis of PC-12 using 85929-5-RR (same clone as 85929-5-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 85929-5-RR (TH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85929-5-PBS in a different storage buffer formulation.

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  WB analysis using 85929-5-RR (same clone as 85929-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85929-5-RR (TH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85929-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85929-5-RR (same clone as 85929-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85929-5-RR (TH antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85929-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85929-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85929-5-RR against Human TH were performed. The affinity constant is 47.4 pM.

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Geprüfte Anwendungen

Produktinformation

85929-5-PBS bindet in WB, IHC, Indirect ELISA TH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TH fusion protein Ag23034
• Vollständiger Name: tyrosine hydroxylase
• Berechnetes Molekulargewicht: 528 aa, 59 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC104967
• Gene symbol: TH
• Gene ID (NCBI): 7054
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons.