CD141/Thrombomodulin Monoklonaler Antikörper

CD141/Thrombomodulin Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5A4C5

Kat-Nr. : 67831-1-PBS

Synonyme

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

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  WB analysis using 67831-1-Ig (same clone as 67831-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67831-1-Ig (same clone as 67831-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67831-1-Ig (THBD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67831-1-Ig (same clone as 67831-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67831-1-Ig (same clone as 67831-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67831-1-Ig (same clone as 67831-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67831-1-Ig (CD141/Thrombomodulin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67831-1-Ig (same clone as 67831-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67831-1-Ig (CD141/Thrombomodulin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67831-1-Ig (same clone as 67831-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67831-1-Ig (CD141/Thrombomodulin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67831-1-PBS bindet in WB, IHC, Indirect ELISA CD141/Thrombomodulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD141/Thrombomodulin fusion protein Ag5723
• Vollständiger Name: thrombomodulin
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC035602
• Gene symbol: THBD
• Gene ID (NCBI): 7056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thrombomodulin, also known as THBD and CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).