THEM2 Polyklonaler Antikörper

THEM2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16782-1-PBS

Synonyme

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  WB analysis using 16782-1-AP (same clone as 16782-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16782-1-AP (THEM2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 16782-1-AP (same clone as 16782-1-PBS)

  IP result of anti-THEM2 (IP:16782-1-AP, 4ug; Detection:16782-1-AP 1:2000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 16782-1-AP (same clone as 16782-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16782-1-AP (THEM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 16782-1-AP (same clone as 16782-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16782-1-AP (THEM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16782-1-AP (same clone as 16782-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using THEM2 antibody (16782-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16782-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16782-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA THEM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: THEM2 fusion protein Ag10212
• Vollständiger Name: thioesterase superfamily member 2
• Berechnetes Molekulargewicht: 140 aa, 15 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC000894
• Gene symbol: THEM2
• Gene ID (NCBI): 55856
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Them2 (also named Acot13) is a homotetrameric fatty acyl-CoA thioesterase. Them2 is enriched in liver and oxidative tissues, including BAT, and is primarily associated with mitochondria.