THOC2 Polyklonaler Antikörper

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• KD/KO Validated

THOC2 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 55178-1-AP

Synonyme

Tho2, THO complex subunit 2, THO complex 2, hTREX120, CXorf3

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  WB analysis of HeLa using 55178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55178-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 55178-1-AP

  IP result of anti-THOC2 (IP:55178-1-AP, 4ug; Detection:55178-1-AP 1:600) with HeLa cells lysate 1600 ug.

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  IF Staining of HeLa using 55178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THOC2 antibody (55178-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HeLa using 55178-1-AP

  Immunofluorescent analysis of HeLa cells using 55178-1-AP (THOC2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

55178-1-AP bindet in WB, IF/ICC, IP, ELISA THOC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: THO complex 2
• Berechnetes Molekulargewicht: 183 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: NM_020449
• Gene symbol: THOC2
• Gene ID (NCBI): 57187
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

THOC2 also termed as THO complex 2 or CXorf3 is 1593 amino acid protein, which belongs to the THOC2 family. THOC2 is a component of heteromultimeric THO/TREX (transcription/export) complex, which is a multi-protein structure consisting of THOC1, THOC2, THOC3, BAT1 and ALY. The TREX complex associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism.

Publikationen

Species	Application	Title
human	WB	Hum Mol Genet; Clinical and functional characterization of recurrent missense variants implicated in THOC6-related intellectual disability.; Authors - Mattioli Francesca
human	WB	Viruses; Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts.; Authors - Toshie Sakuma ; KD Validated
human	WB	Mol Cell; YTHDC1 cooperates with the THO complex to prevent RNA-damage-induced DNA breaks; Authors - Ning Tsao
human	WB	Int J Biol Macromol; Targeting mRNA export complex macromolecules THO subunits (Thoc2 and Thoc5) for somatic cell reprograming; Authors - Abdur Rehman