THOC2 Polyklonaler Antikörper

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• KD/KO Validated

THOC2 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55178-1-PBS

Synonyme

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  WB analysis of HeLa using 55178-1-AP (same clone as 55178-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 55178-1-AP (same clone as 55178-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 55178-1-AP (same clone as 55178-1-PBS)

  IP result of anti-THOC2 (IP:55178-1-AP, 4ug; Detection:55178-1-AP 1:600) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 55178-1-AP (same clone as 55178-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THOC2 antibody (55178-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 55178-1-AP (same clone as 55178-1-PBS)

  Immunofluorescent analysis of HeLa cells using 55178-1-AP (THOC2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 55178-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55178-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA THOC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: THO complex 2
• Berechnetes Molekulargewicht: 183 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: NM_020449
• Gene symbol: THOC2
• Gene ID (NCBI): 57187
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

THOC2 also termed as THO complex 2 or CXorf3 is 1593 amino acid protein, which belongs to the THOC2 family. THOC2 is a component of heteromultimeric THO/TREX (transcription/export) complex, which is a multi-protein structure consisting of THOC1, THOC2, THOC3, BAT1 and ALY. The TREX complex associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism.