THOC5 Polyklonaler Antikörper

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• KD/KO Validated

THOC5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14862-1-AP

Synonyme

C22orf19, Functional spliceosome-associated protein 79, KIAA0983

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  WB analysis using 14862-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14862-1-AP (THOC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14862-1-AP

  WB result of THOC5 antibody (14862-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THOC5 transfected HEK-293 cells.

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  IP experiment of HEK-293 using 14862-1-AP

  IP result of anti-THOC5 (IP:14862-1-AP, 4ug; Detection:14862-1-AP 1:5000) with HEK-293 cells lysate 920 ug.

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  IHC staining of human lung cancer using 14862-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14862-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14862-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14862-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14862-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14862-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using THOC5 antibody (14862-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below

Produktinformation

14862-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA THOC5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: THOC5 fusion protein Ag6664
• Vollständiger Name: THO complex 5
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC003615
• Gene symbol: THOC5
• Gene ID (NCBI): 8563
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

THOC5, also named as C22orf19, or KIAA0983, is a 683 amino acid protein, which belongs to the THOC5 family. THOC5 is ubiquitously expressed in many tissues. THOC5 localizes in the nucleus and acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA.THOC5 plays a role in cytokine-stimulated monocytic development and affects granulocyte/macrophage differentiation.

Publikationen

Species	Application	Title
mouse		Cell Stem Cell; The THO Complex Regulates Pluripotency Gene mRNA Export and Controls Embryonic Stem Cell Self-Renewal and Somatic Cell Reprogramming.; Authors - Wang Li L ; KD Validated