THOP1 Rekombinanter Antikörper

THOP1 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241607G8

Kat-Nr. : 84319-3-PBS

Synonyme

241607G8, EC:3.4.24.15, EP24.15, MEPD_HUMAN, MP78

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  WB analysis using 84319-3-RR (same clone as 84319-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84319-3-RR (THOP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84319-3-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01191-3

  Sandwich ELISA standard curve of MP01191-3, Human THOP1 Recombinant Matched Antibody Pair - PBS only. 84319-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2268. 84319-3-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

• 

  Sample test of MP01191-3

  The mean THOP1 concentration was determined to be 154.80 ng/mL in THP-1 cell extract based on a 4.00 mg/mL extract load.

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  Cytometric bead array standard curve of MP01191-3

  Cytometric bead array standard curve of MP01191-3, THOP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84319-2-PBS. Detection antibody: 84319-3-PBS. Standard: Ag2268. Range: 0.313-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 84319-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84319-3-RR against Human THOP1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84319-3-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test THOP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: THOP1 fusion protein Ag2268
• Vollständiger Name: thimet oligopeptidase 1
• Berechnetes Molekulargewicht: 689 aa, 79 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC000135
• Gene symbol: THOP1
• Gene ID (NCBI): 7064
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thimet oligopeptidase(THOP1) is a metallopeptidase implicated in the metabolism of many sleep-related peptides, including angiotensin I, gonadotropin releasing hormone (GnRH), neurotensin, and opioid peptides