THRA Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

THRA Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1A11C8

Kat-Nr. : 66703-1-Ig

Synonyme

1A11C8, AR7, c-erbA-1, c-erbA-alpha, EAR 7

---

• 

  WB analysis of A431 using 66703-1-Ig

  WB result of THRA antibody (66703-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THRA transfected A431 cells.

• 

  IHC staining of mouse brain using 66703-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66703-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 66703-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 66703-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 66703-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 66703-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 66703-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using THRA antibody (66703-1-Ig, Clone: 1A11C8 ) at dilution of 1:1300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Schilddrüsenkarzinomgewebe, Mausherzgewebe, Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:650-1:2600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

66703-1-Ig bindet in WB, IHC, IF/ICC, ELISA THRA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: THRA fusion protein Ag27189
• Vollständiger Name: thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)
• Berechnetes Molekulargewicht: 490 aa, 54 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC002728
• Gene symbol: THRA
• Gene ID (NCBI): 7067
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Mol Cell Endocrinol; Exogenous Thyroxine Increases Cardiac GLUT4 Translocation in Insulin Resistant OLETF Rats; Authors - Dora A Mendez
pig	WB	Theriogenology; Thyroxine regulates pig Sertoli cell line proliferation and maturation through the IKK/NFκB and p38 MAPK signaling pathways; Authors - Jing Li
human	WB	Adv Sci (Weinh); A NRF2 Regulated and the Immunosuppressive Microenvironment Reversed Nanoplatform for Cholangiocarcinoma Photodynamic-Gas Therapy; Authors - Weimin Wang
mouse	WB	J Muscle Res Cell Motil; RNA-seq and ChIP-seq unveils thyroid hormone receptor α deficiency affects skeletal muscle myoblast proliferation and differentiation via Col6a1 during aging; Authors - Runqing Shi