THRAP3 Polyklonaler Antikörper

THRAP3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 19744-1-AP

Synonyme

THRAP3, TRAP150

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Galerie der Validierungsdaten

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  WB analysis using 19744-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19744-1-AP (THRAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19744-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19744-1-AP (THRAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19744-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19744-1-AP (THRAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19744-1-AP

  IP Result of anti-THRAP3 (IP:19744-1-AP, 4ug; Detection:19744-1-AP 1:500) with HeLa cells lysate 600ug.

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  IHC staining of human ovary tumor using 19744-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19744-1-AP (THRAP3 Antibody) at dilution of 1:50.

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  IHC staining of human thyroid cancer using 19744-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 19744-1-AP (THRAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 19744-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19744-1-AP (THRAP3 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 19744-1-AP

  Immunofluorescent analysis of HepG2 cells using 19744-1-AP (THRAP3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, LNCaP-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

19744-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA THRAP3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: thyroid hormone receptor associated protein 3
• Berechnetes Molekulargewicht: 109 kDa
• Beobachtetes Molekulargewicht: 109-130 kDa
• GenBank-Zugangsnummer: NM_005119
• Gene symbol: THRAP3
• Gene ID (NCBI): 9967
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

THRAP3, also named as TRAP150, plays a role in transcriptional coactivation. The antibody recognizes the C-term of THRAP3.

Publikationen

Species	Application	Title
pig	WB	Nucleic Acids Res; The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.; Authors - Xin Zong
	IF	J Cell Sci; Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.; Authors - Valentina Iadevaia
human	WB	Cancer Res; NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer; Authors - Ruihui Xie
human	WB,CoIP	Mol Cell Proteomics; Deciphering the clinical significance and kinase functions of GSK3α in colon cancer by proteomics and phosphoproteomics; Authors - Li Gao