Thyroid Hormone Receptor beta Rekombinanter Antikörper

Thyroid Hormone Receptor beta Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240619E7

Kat-Nr. : 83866-2-PBS

Synonyme

THRB, THR1, NR1A2, ERBA2, c-erbA-beta

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  WB analysis of mouse pancreas using 83866-2-RR (same clone as 83866-2-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 83866-2-RR (Thyroid Hormone Receptor beta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83866-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83866-2-RR (same clone as 83866-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Thyroid Hormone Receptor beta antibody (83866-2-RR, Clone: 240619E7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83866-2-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83866-2-RR (same clone as 83866-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Thyroid Hormone Receptor beta Recombinant Antibody (83866-2-RR, Clone:240619E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83866-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83866-2-PBS bindet in WB, IF/ICC, FC (Intra), ELISA Thyroid Hormone Receptor beta und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Thyroid Hormone Receptor beta fusion protein Ag16276
• Vollständiger Name: thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian)
• Berechnetes Molekulargewicht: 476 aa, 54 kDa
• Beobachtetes Molekulargewicht: 53 kDa and 54 kDa
• GenBank-Zugangsnummer: BC106929
• Gene symbol: Thyroid Hormone Receptor beta
• Gene ID (NCBI): 7068
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thyroid hormone receptor beta (THRB), also known as ERBA2, NR1A2, and THR1, belongs to the nuclear hormone receptor family and NR1 subfamily. THRB is a nuclear hormone receptor that can act as a repressor or activator of transcription. Moreover, THRB is also a high-affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. THRB is the most abundant THR isoform in the liver but is also expressed in other tissues and mediates the effects of THs on hepatic carbohydrate and lipid metabolism (PMID: 34884910). THRB has 2 isoforms with the molecular mass of 53 and 54 kDa (refer to UniProt).