THRSP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

THRSP Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13054-1-AP

Synonyme

S14, Spot 14 protein, SPOT14, THRSP

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse liver using 13054-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13054-1-AP (THRSP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13054-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13054-1-AP (THRSP Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13054-1-AP

  IP Result of anti-THRSP (IP:13054-1-AP, 4ug; Detection:13054-1-AP 1:300) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of human liver using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:100-1:600
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

13054-1-AP bindet in WB, IP, IHC, ELISA THRSP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: THRSP fusion protein Ag3721
• Vollständiger Name: thyroid hormone responsive (SPOT14 homolog, rat)
• Berechnetes Molekulargewicht: 146 aa, 17 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC031989
• Gene symbol: THRSP
• Gene ID (NCBI): 7069
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

THRSP (Thyroid hormone-inducible hepatic protein)  is a protein that in humans is encoded by the THRSP gene. The protein encoded by this gene is similar to the gene product of S14, a rat gene whose expression is limited to liver and adipose tissue and is controlled by nutritional and hormonal factors. This gene has been shown to be expressed in liver and adipocytes, particularly in lipomatous modules. It is also found to be expressed in lipogenic breast cancers, which suggests a role in controlling tumor lipid metabolism (RefSeq). This antibody works well in IHC, WB and IF.

Publikationen

Species	Application	Title
human	WB	Mol Med; Insulin-inducible THRSP maintains mitochondrial function and regulates sphingolipid metabolism in human adipocytes.; Authors - Maria A Ahonen
human	WB	Sci Rep; Elevated serum S14 levels are associated with more severe liver steatosis by ultrasonography.; Authors - Wen-Ti Lin
mouse	WB	Mol Cell Endocrinol; MiR-451a attenuates free fatty acids-mediated hepatocyte steatosis by targeting the thyroid hormone responsive spot 14 gene.; Authors - Ni Zeng ; KD Validated
		Medicine (Baltimore); Serum Spot 14 concentration is negatively associated with thyroid-stimulating hormone level.; Authors - Yen-Ting Chen
		PLoS One; The serum level of a novel lipogenic protein Spot 14 was reduced in metabolic syndrome.; Authors - Yen-Ting Chen