THUMPD1 Polyklonaler Antikörper

THUMPD1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 14921-1-AP

Synonyme

THUMP domain-containing protein 1, THUMPD 1

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  WB analysis using 14921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14921-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14921-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14921-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14921-1-AP (THUMPD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14921-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14921-1-AP (THUMPD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 14921-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using THUMPD1 antibody (14921-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, MCF-7-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14921-1-AP bindet in WB, IHC, IF/ICC, ELISA THUMPD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: THUMPD1 fusion protein Ag6739
• Vollständiger Name: THUMP domain containing 1
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC000448
• Gene symbol: THUMPD1
• Gene ID (NCBI): 55623
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Death Differ; Lactylation of NAT10 promotes N4-acetylcytidine modification on tRNASer-CGA-1-1 to boost oncogenic DNA virus KSHV reactivation; Authors - Qin Yan
human	IF	Biosci Rep; Pan-cancer analysis of N4-acetylcytidine adaptor THUMPD1 as a predictor for prognosis and immunotherapy.; Authors - Kuangxun Li
human	IHC	iScience; RNA modification-related genes illuminate prognostic signature and mechanism in esophageal squamous cell carcinoma; Authors - Hui Han