THYN1 Polyklonaler Antikörper

THYN1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15867-1-AP

Synonyme

HSPC144, MDS012, My0054, MY105, THY28, THY28KD, thymocyte nuclear protein 1, Thymocyte protein Thy28, THYN1

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Galerie der Validierungsdaten

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  WB analysis using 15867-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15867-1-AP (THYN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15867-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15867-1-AP (THYN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15867-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15867-1-AP (THYN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 15867-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15867-1-AP (THYN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15867-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15867-1-AP (THYN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15867-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15867-1-AP (THYN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15867-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15867-1-AP (THYN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15867-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15867-1-AP (THYN1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15867-1-AP bindet in WB, IHC, IF,ELISA THYN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: THYN1 fusion protein Ag8481
• Vollständiger Name: thymocyte nuclear protein 1
• Berechnetes Molekulargewicht: 225aa,26 kDa; 166aa,19 kDa
• Beobachtetes Molekulargewicht: 28-32 kDa
• GenBank-Zugangsnummer: BC006978
• Gene symbol: THYN1
• Gene ID (NCBI): 29087
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

THYN1, also named as THY28, is a 28-32 kDa protein which located mainly in the nucleus. It appears to correlate with induction of apoptosis. (PMID:14601557)