TIA1 Rekombinanter Antikörper

TIA1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3N22

Kat-Nr. : 82672-1-PBS

Synonyme

3N22, Cytotoxic granule associated RNA binding protein TIA1, Nucleolysin TIA-1 isoform p40, p40 TIA 1, p40-TIA-1

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  WB analysis using 82672-1-RR (same clone as 82672-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82672-1-RR (TIA1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82672-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 82672-1-RR (same clone as 82672-1-PBS)

  WB result of TIA1 antibody (82672-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIA1 transfected Jurkat cells. This data was developed using the same antibody clone with 82672-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82672-1-RR (same clone as 82672-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using TIA1 antibody (82672-1-RR, Clone: 3N22 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82672-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82672-1-RR (same clone as 82672-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using TIA1 antibody (82672-1-RR, Clone: 3N22 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82672-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82672-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82672-1-RR against Human TIA1 were performed. The affinity constant is 0.385 nM.

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Geprüfte Anwendungen

Produktinformation

82672-1-PBS bindet in WB, IF/ICC, Indirect ELISA TIA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TIA1 fusion protein Ag2778
• Vollständiger Name: TIA1 cytotoxic granule-associated RNA binding protein
• Berechnetes Molekulargewicht: 214 aa, 24 kDa, 43 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC015944
• Gene symbol: TIA1
• Gene ID (NCBI): 7072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1 mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43.