TIA1 Rekombinanter Antikörper

TIA1 Rekombinant Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

3N22

Kat-Nr. : 82672-1-RR

Synonyme

Nucleolysin TIA 1 isoform p40, p40 TIA 1, RNA binding protein TIA 1, TIA 1, TIA1

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Galerie der Validierungsdaten

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  WB analysis using 82672-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82672-1-RR (TIA1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 82672-1-RR

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using TIA1 antibody (82672-1-RR, Clone: 3N22 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 82672-1-RR

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using TIA1 antibody (82672-1-RR, Clone: 3N22 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, COLO 320-Zellen, Jurkat-Zellen, K-562-Zellen, MOLT-4-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF: sodium arsenite treated HeLa cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82672-1-RR bindet in WB, IF, ELISA TIA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TIA1 fusion protein Ag2778
• Vollständiger Name: TIA1 cytotoxic granule-associated RNA binding protein
• Berechnetes Molekulargewicht: 214 aa, 24 kDa, 43 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC015944
• Gene symbol: TIA1
• Gene ID (NCBI): 7072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1 mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43.