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TIAM2 Polyklonaler Antikörper

TIAM2 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26870-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using TIAM2 Polyclonal antibody (26870-1-AP)

    WB analysis using 26870-1-AP (same clone as 26870-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TIAM2 Polyclonal antibody (26870-1-AP)

    WB analysis using 26870-1-AP (same clone as 26870-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using TIAM2 Polyclonal antibody (26870-1-AP)

    IF Staining of RAW 264.7 using 26870-1-AP (same clone as 26870-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TIAM2 antibody (26870-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using TIAM2 Polyclonal antibody (26870-1-AP)

    FC experiment of K-562 using 26870-1-AP (same clone as 26870-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human TIAM2 (26870-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

26870-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA TIAM2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TIAM2 fusion protein Ag25469
Vollständiger Name T-cell lymphoma invasion and metastasis 2
Beobachtetes Molekulargewicht190-200 kDa
GenBank-ZugangsnummerBC137275
Gene symbol TIAM2
Gene ID (NCBI) 26230
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIAM2 is a Rac1-specific GEF, which was identified originally as a homologue of TIAM1. TIAM2 plays a critical role in the malignancy development of many human cancers. TIAM2 expression upregulates N-cadherin and vimentin, thus enhancing epithelial-to-mesenchymal transition (EMT) and leading to the enhancement of proliferation and invasion in hepatoma cells.