TICAM2 Polyklonaler Antikörper

TICAM2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19524-1-AP

Synonyme

MyD88-4, Putative NF-kappa-B-activating protein 502, TICAM 2, TICAM-2, TIR domain-containing adapter molecule 2

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  WB analysis of PC-3 using 19524-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19524-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 19524-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19524-1-AP (TICAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19524-1-AP (TICAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate hyperplasia using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 19524-1-AP (TICAM2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 19524-1-AP (TICAM2 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, humanes Hodengewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Prostatahyperplasie-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19524-1-AP bindet in WB, IHC, ELISA TICAM2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: toll-like receptor adaptor molecule 2
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: NM_021649
• Gene symbol: TICAM2
• Gene ID (NCBI): 353376
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TICAM2, also named TIRAP3, TIRP, and TRAM, is involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. TICAM2 is involved in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS.In WB, the MW of TICAM2 is about 27-32 kDa.