TIGAR Polyklonaler Antikörper

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• KD/KO Validated

TIGAR Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 22136-1-AP

Synonyme

C12orf5, TP53-induced glycolysis regulatory phosphatase, TP53-induced glycolysis and apoptosis regulator, Fructose-2,6-bisphosphatase TIGAR, EC:3.1.3.46

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  WB analysis using 22136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22136-1-AP (TIGAR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below

Produktinformation

22136-1-AP bindet in WB, IHC, ELISA TIGAR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TIGAR fusion protein Ag17532
• Vollständiger Name: chromosome 12 open reading frame 5
• Berechnetes Molekulargewicht: 270 aa, 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC012340
• Gene symbol: TIGAR
• Gene ID (NCBI): 57103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The TP53-induced glycolysis and apoptosis regulator (TIGAR), first reported in 2006, is a downstream target gene of p53 and an important factor involved in glycolysis and apoptosis . It is indispensable in metabolism and is involved in metabolic syndrome, including hyperglycemia, insulin resistance, alcoholic fatty liver and tissue ischemia. TIGAR is highly expressed in many cancer cells to promote cell survival.

Publikationen

Species	Application	Title
mouse	WB	Autophagy; TIGAR exacerbates obesity by triggering LRRK2-mediated defects in macroautophagy and chaperone-mediated autophagy in adipocytes; Authors - Tian Zhang
mouse	WB	Free Radic Biol Med; Oxidative stress-induced alterations in retinal glucose metabolism in Retinitis Pigmentosa.; Authors - Yogita Kanan
mouse	WB	Neurobiol Dis; Maternal Herpesviridae infection during pregnancy alters midbrain dopaminergic signatures in adult offspring.; Authors - Amanda R Burmeister
human	WB	Cell Death Dis; MiR-652-5p elevated glycolysis level by targeting TIGAR in T-cell acute lymphoblastic leukemia.; Authors - Shan Liu
mouse	WB	Biochem Biophys Res Commun; Cordycepin exhibits anti-fatigue effect via activating TIGAR/SIRT1/PGC-1α signaling pathway; Authors - Xiaoming Chai
human,mouse	WB	Phytother Res; Dual targeting of wild-type p53 and gut microbiota by Magnolol represses key metabolic process and kills CRC cells; Authors - Haixia Ji