TIGAR Rekombinanter Antikörper

TIGAR Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242625D10

Kat-Nr. : 85052-1-PBS

Synonyme

C12orf5, EC:3.1.3.46, Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TP53-induced glycolysis regulatory phosphatase

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  Sandwich ELISA standard curve of MP01803-1

  Sandwich ELISA standard curve of MP01803-1, Human TIGAR Recombinant Matched Antibody Pair - PBS only. 85052-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag17532. 85052-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP01803-1

  The mean TIGAR concentration was determined to be 17.53 ng/mL in HCT 116 cell extract based on a 1.40 mg/mL extract load, 14.64 ng/mL in A431 cell extract based on a 1.30 mg/mL extract load and 21.52 ng/mL in HepG2 cell extract based on a 1.00 mg/mL extract load.

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  Cytometric bead array standard curve of MP01803-1

  Cytometric bead array standard curve of MP01803-1, TIGAR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85052-1-PBS. Detection antibody: 85052-2-PBS. Standard: Ag17532. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85052-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 85052-1-PBS against Human TIGAR was performed. The affinity constant is 1.98 nM.

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Geprüfte Anwendungen

Produktinformation

85052-1-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test TIGAR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TIGAR fusion protein Ag17532
• Vollständiger Name: chromosome 12 open reading frame 5
• Berechnetes Molekulargewicht: 270 aa, 30 kDa
• GenBank-Zugangsnummer: BC012340
• Gene symbol: TIGAR
• Gene ID (NCBI): 57103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.