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TIGAR Rekombinanter Antikörper

TIGAR Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242625E7

Kat-Nr. : 85052-4-PBS

Synonyme

C12orf5, 242625E7, EC:3.1.3.46, Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator

Filter:
  • Western Blot (WB) analysis of various lysates using TIGAR Recombinant antibody (85052-4-RR)

    WB analysis using 85052-4-RR (same clone as 85052-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85052-4-RR (TIGAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85052-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TIGAR Recombinant antibody (85052-4-RR)

    IHC staining of human colon using 85052-4-RR (same clone as 85052-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85052-4-RR (TIGAR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85052-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TIGAR Recombinant antibody (85052-4-RR)

    IHC staining of human colon using 85052-4-RR (same clone as 85052-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85052-4-RR (TIGAR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85052-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85052-4-RR

    Affinity and Kinetic Characterization of 85052-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85052-4-RR against Human TIGAR were performed. The affinity constant is 0.308 nM.

Geprüfte Anwendungen

Produktinformation

85052-4-PBS bindet in WB, IHC, Indirect ELISA TIGAR und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen TIGAR fusion protein Ag17532
Vollständiger Name chromosome 12 open reading frame 5
Berechnetes Molekulargewicht 270 aa, 30 kDa
Beobachtetes Molekulargewicht 30 kDa
GenBank-ZugangsnummerBC012340
Gene symbol TIGAR
Gene ID (NCBI) 57103
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The TP53-induced glycolysis and apoptosis regulator (TIGAR), first reported in 2006, is a downstream target gene of p53 and an important factor involved in glycolysis and apoptosis . It is indispensable in metabolism and is involved in metabolic syndrome, including hyperglycemia, insulin resistance, alcoholic fatty liver and tissue ischemia. TIGAR is highly expressed in many cancer cells to promote cell survival.