TIGAR Rekombinanter Antikörper

TIGAR Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242625E7

Kat-Nr. : 85052-4-RR

Synonyme

C12orf5, 242625E7, EC:3.1.3.46, Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator

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  WB analysis using 85052-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85052-4-RR (TIGAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 85052-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85052-4-RR (TIGAR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 85052-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85052-4-RR (TIGAR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85052-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85052-4-RR against Human TIGAR were performed. The affinity constant is 0.308 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HCT 116-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85052-4-RR bindet in WB, IHC, ELISA TIGAR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TIGAR fusion protein Ag17532
• Vollständiger Name: chromosome 12 open reading frame 5
• Berechnetes Molekulargewicht: 270 aa, 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC012340
• Gene symbol: TIGAR
• Gene ID (NCBI): 57103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The TP53-induced glycolysis and apoptosis regulator (TIGAR), first reported in 2006, is a downstream target gene of p53 and an important factor involved in glycolysis and apoptosis . It is indispensable in metabolism and is involved in metabolic syndrome, including hyperglycemia, insulin resistance, alcoholic fatty liver and tissue ischemia. TIGAR is highly expressed in many cancer cells to promote cell survival.