TIGIT Rekombinanter Antikörper

TIGIT Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240423D3

Kat-Nr. : 83545-1-PBS

Synonyme

240423D3, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3, VSIG9

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  WB analysis using 83545-1-RR (same clone as 83545-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83545-1-RR (TIGIT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83545-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 83545-1-RR (same clone as 83545-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83545-1-RR (TIGIT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83545-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 83545-1-RR (same clone as 83545-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using TIGIT antibody (83545-1-RR, Clone: 240423D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83545-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 83545-1-RR (same clone as 83545-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TIGIT antibody (83545-1-RR, Clone: 240423D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83545-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83545-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83545-1-RR against Human TIGIT were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83545-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA TIGIT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TIGIT fusion protein Eg0749
• Vollständiger Name: T cell immunoreceptor with Ig and ITIM domains
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: NM_173799.4
• Gene symbol: TIGIT
• Gene ID (NCBI): 201633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).