TIGIT Rekombinanter Antikörper

TIGIT Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240423C7

Kat-Nr. : 98023-2-RR

Synonyme

240423C7, T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3

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  FC experiment of human PBMCs using 98023-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TIGIT Rabbit Recombinant Antibody (98023-2-RR, Clone: 240423C7) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD45RO. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98023-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TIGIT Rabbit Recombinant Antibody (98023-2-RR, Clone: 240423C7) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated.

• 

  Affinity and Kinetic Characterization of 98023-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98023-2-RR against Human TIGIT were performed. The affinity constant is 5.45 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98023-2-RR bindet in FC TIGIT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: T cell immunoreceptor with Ig and ITIM domains
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: NM_173799.4
• Gene symbol: TIGIT
• Gene ID (NCBI): 201633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).