TIGIT Rekombinanter Antikörper

TIGIT Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250731C10

Kat-Nr. : 98026-4-PBS

Synonyme

Vstm3

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  FC experiment of mouse splenocytes using 98026-4-RR (same clone as 98026-4-PBS)

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse TIGIT Rabbit RecAb (98026-4-RR, Clone: 250731C10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L. Cells were then stained with APC Anti-Human CD4. Cells were not fixed. This data was developed using the same antibody clone with 98026-4-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98026-4-RR (same clone as 98026-4-PBS)

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse TIGIT Rabbit RecAb (98026-4-RR, Clone: 250731C10) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse TIGIT Rabbit RecAb (98026-4-RR, Clone: 250731C10) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98026-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98026-4-PBS bindet in FC TIGIT und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DNA
• Vollständiger Name: T cell immunoreceptor with Ig and ITIM domains
• Berechnetes Molekulargewicht: 26kDa
• GenBank-Zugangsnummer: NP_001139797.1
• Gene symbol: Tigit
• Gene ID (NCBI): 100043314
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).