TIGIT Monoklonaler Antikörper

TIGIT Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

1G9

Kat-Nr. : APC-65079

Synonyme

ENSMUSG00000071552, Tigit, Vstm3

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Galerie der Validierungsdaten

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  FC experiment of Babl/c mouse splenocytes using APC-65079

  1X10^6 unstimulated or plate-bound Anti-CD3 and Anti-CD28 stimulated BALB/c mouse splenocytes were surface stained with 0.25 ug CoraLite®488 Anti-Mouse CD3 (CL488-65077, Clone: 17A2) and 0.25 ug APC Anti-Mouse TIGIT (APC-65079, Clone: 1G9). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: plate-bound Anti-CD3 and Anti-CD28 stimulated BALB/c mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-65079 bindet in FC TIGIT und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: T cell immunoreceptor with Ig and ITIM domains
• GenBank-Zugangsnummer: NM_001146325
• Gene symbol: Tigit
• Gene ID (NCBI): 100043314
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).