TIGIT Rekombinanter Antikörper

TIGIT Rekombinant Antikörper für IF-P

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF-P

Konjugation

CoraLite®532 Fluorescent Dye

CloneNo.

240423D3

Kat-Nr. : CL532-83545

Synonyme

VSTM3, VSIG9, V-set and transmembrane domain-containing protein 3, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains

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  IF Staining of mouse spleen using CL532-83545

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite®532 TIGIT antibody (CL532-83545, Clone: 240423D3 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Mausmilzgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL532-83545 bindet in IF-P TIGIT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: T cell immunoreceptor with Ig and ITIM domains
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: NM_173799.4
• Gene symbol: TIGIT
• Gene ID (NCBI): 201633
• Konjugation: CoraLite®532 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 537 nm / 560 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).