TIGIT Rekombinanter Antikörper

TIGIT Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

240242G1

Kat-Nr. : CL594-98026

Synonyme

Vstm3, 240242G1

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  FC experiment of mouse splenocytes using CL594-98026

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 and 0.25 ug CoraLite®594 Anti-Mouse TIGIT Rabbit Recombinant Antibody (CL594-98026, Clone: 240242G1). Cells were not fixed.

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  FC experiment of mouse splenocytes using CL594-98026

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surafce stained with 0.25 ug CoraLite®594 Anti-Mouse TIGIT Rabbit Recombinant Antibody (CL594-98026, Clone: 240242G1) (red), or Isotype Control (blue). 1x10^6 untreated mouse splenocytes were surafce stained with 0.25 ug CoraLite®594 Anti-Mouse TIGIT Rabbit Recombinant Antibody (CL594-98026, Clone: 240242G1) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Annti-CD3/CD28 treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98026 bindet in FC TIGIT und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: T cell immunoreceptor with Ig and ITIM domains
• Berechnetes Molekulargewicht: 26kDa
• GenBank-Zugangsnummer: NP_001139797.1
• Gene symbol: Tigit
• Gene ID (NCBI): 100043314
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).