TIM3 Rekombinanter Antikörper

TIM3 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

5A8

Kat-Nr. : 82836-1-RR

Synonyme

FLJ14428, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIMD 3, TIMD3

---

Galerie der Validierungsdaten

• 

  WB analysis using 82836-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82836-1-RR (TIM3 antibody) at dilution of 1:3500 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, Daudi-Zellen, RAW 264.7-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:7000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82836-1-RR bindet in WB, ELISA TIM3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TIM3 fusion protein Ag2459
• Vollständiger Name: hepatitis A virus cellular receptor 2
• Berechnetes Molekulargewicht: 301 aa, 33 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC020843
• Gene symbol: HAVCR2
• Gene ID (NCBI): 84868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).