TIM22 Polyklonaler Antikörper

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• KD/KO Validated

TIM22 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14927-1-PBS

Synonyme

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  WB analysis using 14927-1-AP (same clone as 14927-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14927-1-AP (TIM22 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 14927-1-AP (same clone as 14927-1-PBS)

  IP result of anti-TIM22 (IP:14927-1-AP, 4ug; Detection:14927-1-AP 1:1000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 14927-1-AP (same clone as 14927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14927-1-AP (TIM22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 14927-1-AP (same clone as 14927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14927-1-AP (TIM22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14927-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14927-1-PBS bindet in WB, IHC, IP, Indirect ELISA TIM22 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TIM22 fusion protein Ag6689
• Vollständiger Name: translocase of inner mitochondrial membrane 22 homolog (yeast)
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC002324
• Gene symbol: TIM22
• Gene ID (NCBI): 29928
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIM22 is a translocase localized in mitochondrial inner membrane. It is implicated in import of nuclear-encoded mitochondrial preproteins to the mitochondria. TIM22 is the essential core of protein insertion complex (TIM22 complex) that consists of TIM22, TIM54, TIM18, and TIM12.